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THE^TM beta Actin Antibody [HRP], mAb, Mouse

Full Name

Abbreviated name-1

THE^TM beta Actin Antibody [HRP], mAb, Mouse

Documents
MSDS: 20081110040350 (PDF)
MSDS: 20090114012119 (PDF)
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Description

GenScript THE^TM beta Actin Antibody [HRP], mAb, Mouse is HRP conjugated THE^TM beta Actin Antibody, mAb, Mouse (A00702) purified from mice ascites by protein G affinity column. This product provides a useful and serves as a specific tool in the study of the intracellular distribution of ß-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP.

Ig Subclass

Mouse IgG2a

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

THE^TM beta Actin Antibody [HRP], mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Clone

2D1D10

Immunogen

A synthetic peptide DDDIAALVVDNGSG coupled to KLH

Purification Method

Protein G affinity column

Reconstitution

Reconstitute the lyophilized antibody (40 µg) with 80 µl deionized water to the final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Fusion Partner

Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.

Conjugation

Horseradish peroxidase (HRP)

Host Species

Mouse

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)

THETM beta Actin Antibody [HRP], mAb, Mouse

THE^TM beta Actin Antibody [HRP], mAb, Mouse