THETM c-Myc Antibody [HRP], mAb, Mouse
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THE TM c-Myc Antibody [HRP], mAb, Mouse  |
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![THE<sup>TM</sup> c-Myc Antibody [HRP], mAb, Mouse](/images/cgi2007/table2.gif "THE<sup>TM</sup> c-Myc Antibody [HRP], mAb, Mouse")
| Full Name |
THETM c-Myc Antibody [HRP], mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-c-Myc [HRP] mAb |
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Description |
GenScript THETM c-Myc Antibody [HRP], mAb, Mouse is immunoaffinity purified from mice ascites with protein A and then conjugated to horseradish peroxidase. Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins. |
Ig Subclass |
Mouse IgG2a |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript THETM c-Myc Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
Clone |
2G8D5 |
Immunogen |
A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.050.2 µg/ml
Western blot: 0.1-1 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized |
Fusion Partner |
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
Conjugation |
Horseradish peroxidase (HRP) |
Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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