| Full Name |
THETM c-Myc Antibody [Biotin], mAb, Mouse
|
Abbreviated name-1 |
Mouse Anti-c-Myc [Biotin] mAb |
|
Description |
GenScript THETM c-Myc Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM Anti-c-Myc Monoclonal Antibody (Mouse) (A00704) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays. |
Ig Subclass |
IgG2a, k |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript THETM c-Myc Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
Clone |
2G8D5 |
Immunogen |
A synthetic peptide EQKLISEEDL coupled to KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody (40 µg) with 80 µl of deionized water (or equivalent) to the final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
|
Fusion Partner |
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells |
Conjugation |
Biotin |
Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
|
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