KT3 Antibody [HRP], pAb, Rabbit
*This product has been discontinued! *
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KT3 Antibody [HRP], pAb, Rabbit  |
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![KT3 Antibody [HRP], pAb, Rabbit](/images/cgi2007/table2.gif "KT3 Antibody [HRP], pAb, Rabbit")
| Full Name |
KT3 Antibody [HRP], pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-KT3 [HRP] pAb; |
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Description |
Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The KT3-Tag is an epitope tag composed of an 11-residue peptide, KPPTPPPEPET, derived from the carboxyl terminal sequence of simian virus 40 (SV40)s large T-antigen. The GenScript Rabbit Anti-KT3-tag [HRP] Polyclonal Antibody is developed in rabbit hosts using a synthetic KT3-tag peptide conjugated to KLH.
GenScript Rabbit Anti-KT3 [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-KT3 [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain KT3-tags. |
Ig Subclass |
Rabbit IgG |
Storage |
GenScript Rabbit Anti-KT3 [HRP] Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
This Antibody recognizes both N-terminal and C-terminal KT3 tagged fusion proteins. |
Concentration |
1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
Immunogen |
KT3 epitope tag peptide KPPTPPPEPET conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized |
Conjugation |
Horseradish peroxidase (HRP) |
Host Species |
Rabbit |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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