| Full Name |
E2-tag Antibody [HRP], pAb, Rabbit
|
Abbreviated name-1 |
Rabbit Anti-E2-tag [HRP] pAb; |
|
Description |
GenScript Rabbit Anti-E2-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-E2-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain E2-tags. |
Ig Subclass |
Rabbit IgG
|
Storage |
GenScript Rabbit Anti-E2-tag [HRP] Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
GenScript Rabbit Anti-E2-tag [HRP] Polyclonal Antibody recognizes C-terminal, N-terminal, and internal E2 tagged fusion proteins. |
Concentration |
1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
Immunogen |
E2 epitope tag peptide GVSSTSSDFRDR conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized |
Conjugation |
Horseradish peroxidase (HRP) |
Host Species |
Rabbit |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
|
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