| Full Name |
Calnexin Antibody, pAb, Rabbit
|
Abbreviated name-1 |
Rabbit anti Calnexin Polyclonal Antibody |
|
Description |
Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. As a chaperone protein, it assists in protein assembly by ensuring that only the properly folded protein is progresses along the secretory pathway. It seems to play a major role in the quality control apparatus of the ER by retaining incorrectly folded proteins.
GenScript Rabbit Anti-Calnexin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 250-300 of human calnexin protein (Swiss Prot: P27824). |
Ig Subclass |
Rabbit IgG |
Storage |
GenScript Rabbit Anti-Calnexin Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
GenScript Rabbit Anti-Calnexin Polyclonal Antibody detects endogenous levels of human calnexin protein. Sequence homology predicts that it will also react with mouse and rat calnexin. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH-coupled) within residues 250-300 of human calnexin (Swiss Prot: P27824) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder (40µg) with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
90 kD |
Observed Band Size |
85 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human. This product has not yet been tested with other species. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
MSDS: 20081127021427 (PDF)
