Calnexin Antibody, pAb, Rabbit
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Calnexin Antibody, pAb, Rabbit  |
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| Full Name |
Calnexin Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-Calnexin pAb |
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Description |
Calnexin is calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.
GenScript Calnexin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide from carboxyl terminal of human calnexin protein (Swiss Prot: P27824). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Calnexin Antibody, pAb, Rabbit detects endogenous levels of human and mouse calnexin protein. Sequence homology predicts that it will also react with rat, bovine, canine, and Sumatran orangutan calnexin. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH-coupled) from carboxyl terminal of human calnexin (Swiss Prot: P27824). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
90 kD |
Observed Band Size |
85 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human and mouse. This product has not yet been tested with other species. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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MSDS: 20081229202050 (PDF)
