| Full Name |
Calbindin Antibody, pAb, Rabbit
|
Abbreviated name-1 |
Rabbit Anti-Calbindin Polyclonal Antibody |
|
Description |
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27 kD protein induced by vitamin D in the duodenums of young birds. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains four active calcium-binding domains and two modified domains that presumably have lost their calcium-binding capacity. The neurons in the brains of patients with Huntingtons disease are calbindin-depleted.
GenScript Rabbit Anti-Calbindin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from residues 150-200 of human calbindin protein (Swiss Prot: P05937). |
Ig Subclass |
Rabbit IgG |
Storage |
GenScript Rabbit Anti-Calbindin Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
GenScript Rabbit Anti-Calbindin Polyclonal Antibody detects human calbindin protein. Sequence homology predicts that it will also to react with mouse and rat calbindin. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from residues 150-200 of human calbindin protein (Swiss Prot: P05937) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with 80 µl of deionized water (or equivalent) to antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
IHC: 5-10 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human. This product has not yet been tested with other species. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
MSDS: 20090131205137 (PDF)
