Doublecortin Antibody, pAb, Rabbit
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Doublecortin Antibody, pAb, Rabbit  |
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| Full Name |
Doublecortin Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-Doublecortin pAb |
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Description |
Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. It seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Doublecortin protein may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. As a cytoskeletal protein which affects neuronal migration by regulating the stability of microtubules, Doublecortin protein may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. Doublecortin is likely to work with LIS-1 in an overlapping, but distinct, signaling pathway that promotes neuronal migration.
GenScript Doublecortin Antibody, pAb, Rabbit is developed in Rabbit using a KLH-coupled synthetic peptide from within residues 230-280 of human Doublecortin protein (Swiss Prot: O43602). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Doublecortin Antibody, pAb, Rabbit detects endogenous levels of human, mouse and rat Doublecortin protein. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from within residues 230-280 of human Doublecortin protein (Swiss Prot: O43602). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder (40 µg) with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
49 kD |
Observed Band Size |
49 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse and rat. Reactivity to other species is not tested yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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MSDS: 20090410035007 (PDF)
