GFAP Antibody, pAb, Rabbit
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GFAP Antibody, pAb, Rabbit  |
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| Full Name |
GFAP Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-GFAP pAb |
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Description |
GFAP (Glial fibrillary acidic protein) is a member of intermediate filament (IF) protein family which consists mainly of cytokeratin, GFAP, desmin, and vimentin. Like other IF proteins, GFAP is composed of an amino terminal head domain, a central rod domain, and a carboxyl terminal tail domain. The amino terminal region of GFAP shares considerable sequence divergence with other IF proteins, and probably participates in mediation of the tissue specific functions. GFAP intermediate filament serves as a useful marker of astroglia in the brain since it is specifically expressed in astrocytes and certain other astroglia. This protein may be required for communications between Bergmann glia and Purkinje cells during long-term depression induction and maintenance.
GenScript Rabbit Anti-GFAP Polyclonal Antibody is developed in rabbit using a synthetic KLH-coupled peptide from within 250-300 of human GFAP (Swiss Prot: P14136).
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Ig Subclass |
Rabbit IgG |
Storage |
GenScript Rabbit Anti-GFAP Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
GenScript Rabbit Anti-GFAP Polyclonal Antibody detects endogenous levels of human and rat GFAP. It is predicted to react with sumatran orangutan GFAP protein according to sequence homology. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled peptide from within residues 250-300 of human GFAP. (Swiss Prot: P14136) |
Reconstitution |
Reconstitute the lyophilized powder with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Other Applications: user-optimized
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Species Reactivity |
Human and rat. Reactivity to other species is not tested yet. |
Host Species |
Rabbit |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
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MSDS: 20090410041307 (PDF)
