NSE Antibody, pAb, Rabbit
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NSE Antibody, pAb, Rabbit  |
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| Full Name |
NSE Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-NSE pAb |
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Description |
Enolase is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase has three subunits (alpha, beta and gamma) that can form homo and heterodimers. Homodimers of gamma enolase are neuronal-specific. NSE (neuron specific enolase) is expressed primarily in neurons. It is also found in elevated levels in plasma in certain neoplasias including pediatric neuroblastoma and small cell lung cancer. NSE is widely used as a diagnostic marker in a variety of clinical assays.
GenScript Rabbit Anti-NSE Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human NSE (Swiss Prot: P09104). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-NSE Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSE protein. It may cross-reacts with beta enolase according to sequence homology. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from N-terminal of human NSE (Swiss Prot: P09104). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Other Applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse, and rat. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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Related Products
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MSDS: 20090525220001 (PDF)
