| Full Name |
Myelin Basic Protein Antibody, pAb, Rabbit
|
Abbreviated name-1 |
Rabbit Anti-Myelin Basic Protein pAb |
|
Description |
Myelin basic protein (MBP) participates in the process of myelination and has a role in both its formation and stabilization. Alternative splice combined with post-translational modifications give rise to a wide spectrum of isomers, with each of them potentially having a particular function. The MBP classic group isoforms (isoforms 4 to 14) are the most abundant protein components of the myelin membrane in CNS. MBP phosphorylation at threonine 125 modulates the contribution of MBP to the stability of the myelin sheath. Myelin basic protein serves as an oligodendrocyte marker in neuroscience study.
GenScript Rabbit Anti-Myelin Basic Protein Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human Myelin Basic Protein (Swiss Prot: P02686). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Myelin Basic Protein Polyclonal Antibody detects endogenous levels of mouse and rat Myelin Basic Protein. It is predicted to react with human Myelin Basic Protein according to sequence homology. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from C-terminal of human Myelin Basic Protein (Swiss Prot: P02686). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized. |
Conjugation |
Unconjugated |
Species Reactivity |
Mouse and rat. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
|
MSDS: 20090525043557 (PDF)
