| Full Name |
GAP-43 Antibody, pAb, Rabbit
|
Abbreviated name-1 |
GAP 43 antibody |
|
Description |
GAP-43 has been termed as a growth or plasticity protein, because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is considered to be a crucial component involved in effective regenerative response in nervous system. Its phosphorylation by protein kinase C correlates with long-term potentiation. It is used widely to specifically label injured neurons and to score neuronal regeneration.
GenScript Rabbit Anti-GAP-43 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human GAP-43 (Swiss Prot: P17677).
|
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-GAP-43 Polyclonal Antibody detects endogenous levels of mouse and rat GAP-43. It is predicted to react with human GAP-43 according to sequence homology.
Positive Control: Mouse brain and rat brain lysates
|
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
Immunogen |
KLH-coupled synthetic peptide from C-terminal of human GAP-43 (Swiss Prot: P17677). |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized
|
Conjugation |
Unconjugated |
Species Reactivity |
Mouse and rat. This product has not been tested with other species yet. |
Host Species |
Rabbit |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
|
MSDS: 20090614232945 (PDF)
