| Full Name |
TMB |
Abbreviated Name-1 |
|
|
Description |
3,3',5,5'-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negtive) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidase. |
Synonyms |
3,3',5,5'-Tetramethylbenzidine; [1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-; TMBZ |
Molecular Formula |
C16H20N2 |
Molecular Weight |
240.3 |
Appearance |
White or pale grayish brown crystalline powder. |
Storage |
2-8°C, protect from metal, moisture, and light. |
Shipping Condition |
Ambient temperature. |
| Specification |
|---|
Assay |
≥99% (titr.) |
Melting Point |
166 °C |
Boiling Point |
168 - 169 °C |
Residue On Ignition |
≤0.2% |
Loss on Drying |
≤0.5% |
Solubility |
5%, ethyl acetate, clear to slightly hazy colorless to yellow, yellow-green or orange solution. |
|
MSDS: 20081119214610 (PDF)
