酵母発現系
GenScript's 酵母発現系 combines the best features of bacterial and eukaryotic expression systems. It is ideal for the large-scale production of recombinant eukaryotic proteins. Our 酵母発現系 incorporates high yield, high productivity, chemically defined media, and mammalian-like product processing with stable and durable production strains to dramatically lower protein production costs.
GenScript employs a transgenic yeast strain to glycosylate recombinant proteins in the same manner as mammalian cells, making it practical to produce proteins with increased applicability in human therapies. This yeast expression system also provides creative and reliable approach to the expression of proteins that are difficult or impossible to express in other systems.
Advantages of Yeast Expression:
- Rapid transient expression
- Superior expression level
- Controllable process
- Mammalian-like post-translational modification
|
- Generations of stability
- Durability
- Cost-effective expression
- Maximum value
|
Services Included:
- Subcloning of the gene of interest into a yeast expression vector
- Transformation of yeast and selection of transformants
- Screening of positive transformants by PCR and verification integration of the gene
- Verification of expression of the gene of interest by blot analysis
- Pilot expression analysis and optimization
- Optimization of expression and fermentation
- Small-scale expression from one to five liters
- Scale-up expression and fermentation
- 15N- or 13C-labeled protein expression
Service Specifications:
| Services | Description | Deliverables |
| I. Generation of recombinant yeast vector |
- Amplification/isolation of target gene or synthetic gene of interest and cloning into yeast expression vector
- Transformation of the expression clone into yeast and selection of transformants
|
- Expression vector with insert (5 µg)
|
| II. Pilot expression analysis and optimization |
- Screening of positive transformants by PCR to verify gene integration
- Testing for expression of the recombinant protein by western blot analysis
|
|
| III. Small-scale expression & purification |
- Verification of gene expression by SDS-PAGE and western analysis
- Small-scale expression from one to five liters
|
- Cell pellet or protein
- QC data
|
| IV. Large-scale protein production and purification |
- Large-scale production up to 500 L
- Protein purification by affinity column, ion exchange, gel filtration, and hydrophobic column
- Removal of tags
|
- Yeast paste, from 10 L to 500 L
- Purified proteins
- QC data
|
お見積り依頼及びご注文：
 お見積りをご依頼する際には、Quotation/Order Formをダウンロードし、必要事項をご記入のうえ、電子メール又はファクスでご送信ください。
ご注文については、Quotation/Order Formをダウンロードし、必要事項をご記入のうえ、弊社の注文書PO（Purchase Order）或いはクレジットカード情報と共に電子メール又はファクスにてご送信ください。クレジットカード情報は電話または弊社のsecure online messaging systemにて提出することもできます。
お客様のサンプルをご提出の際には、必要事項ご記入済のQuotation/Order Formの複写本をサンプルと同封し以下の宛先まで郵送してください：Protein Services, GenScript, 860 Centennial Ave., Piscataway, NJ 08854, US.
ご質問またはプロジェクト進行状況に関するお問い合わせは、電子メール電話、ファックス、または弊社の secure online messaging systemで受け付けております。
月曜日から金曜日までの営業日において、弊社では24時間サービスを提供しています。
 |
電子メールでのご注文: japanmarket@genscript.com |
 |
電話でのご注文: 81-3-6206-8985(Japanese and Korean),1-732-885-9188(English) |
 |
ファックスでのご注文: 81-3-6206-8987(Japanese and Korean), 1-732-210-0262, 1-732-885-5878(English) |
|
