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E00011
 Uracil DNA Glycosylase
2000 U
$145
E00011D10
 Uracil DNA Glycosylase (10 U/µl)
2000 U

Uracil DNA Glycosylase


Description
E. coli Uracil DNA Glycosylase (UNG) catalyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).
Source
An E. coli strain that carries the UNG gene from E. coli.
Applications
  • Glycosylase mediated single nucleotide polymorphism detection (GMPD)
  • Site-directed mutagenesis
  • As a probe for protein-DNA interaction studies
  • Rapid and efficientcloning of PCR products
  • Elimination carry-over contamination in PCR

Unit Definition
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Uracil DNA Glycosylase Reaction Buffer with 1 unit or uracil DNA Glycosylase and 0.2 μg [3H]-uracil DNA (104-105 cpm/μg).
10X UNG Reaction Buffer
200 mM Tris-HCl, pH 8.0 (at 25°C), 10 mM Dithiothreitol, 10 mM EDTA.
Reaction Conditions
1X UNG Reaction Buffer, incubate at 37°C.
Inhibition and Inactivation
Inactivated by heating at 95°C for 10 min. Enzyme activity is partially restored at temperatures lower than 55°C.
Quality Control
Activity, SDS-PAGE (purity), 16-hour incubation, exonuclease and endonuclease activity Store at -20.000 C


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