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E00012
 Taq DNA Polymerase, concentrated
2500 U
$149


Taq DNA Polymerase, concentrated


Key Features
  • Terminal Transferase Activity: A single nucleotide (adenosine) is added to the 3′ end of the extension product.
  • High-purity: No contamination activity has been detected in standard test reactions.

Description
Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used in PCR*. In some cases, such as RAPD PCR, adding large volume of general Taq DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated Taq DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency.

  • Note: Concentrated Taq DNA Polymerase (GenScript, E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride. The dNTP (10 mM) mixture may be ordered separately (See related products).

    Applications
    The applications of Taq DNA polymerase are as follows:
  • PCR*
  • 3'A-tailing of blunt ends
  • Primer extension
  • DNA sequencing

    Formulation:
    Genscript Taq DNA Polymerase has been formulated using GenScript's proprietary technology. The enzyme can be shipped at room temperature or stored at 37°C for seven days without any significant loss of activity.

    Unit Definition
    one unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

    10 X Reaction Buffer (with Mg2+)
    500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 15 mM MgCl2, 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs.

    Important: If another reaction buffers are used with Taq DNA Polymerase, Triton X-100 must be added to a final concentration of 0.1% to ensure high enzyme activity with Taq DNA Polymerase;

    Concentration
    Supplied in 25 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol.

    QC Tests
    PCR* performance, activity, nuclease
    Lane Taq Amount Used
    1Leading
    Brand A    		0.1
    2 0.25
    3 0.5
    4Leading
    Brand B    		0.1
    5 0.25
    6 0.5
    7GenScript0.1
    8 0.25
    9 0.5


    Store at -20.000 C


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