Protein A Resin

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Description

GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg Porcine IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.

Total Volume

5 ml settled resin (10 ml 50% slurry)

Key Features

High IgG-binding capacity
Milder elution condition than protein G resin
Resin is reusable up to 10 times with no significant loss of binding capacity.

Characteristics:

Ligand	Recombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand	5
MW of ligand	Approximately 34 kDa
PI of ligand	5.17
Degree of substitution	Approximately 2 mg protein A/ml settled resin
Static binding capacity	>20 mg porcine IgG/ml settled resin
Matrix spherical	agarose, 4% cross-linked
Average particle size	90 μm (45-165 μm)
Storage solution	1X PBS containing 20% ethanol
Storage & Stability	12 months when stored unopened at 2-8℃

Technical Manual

Components

Application	HA-tag Detection
Pretreat A solution	100 ml
Pretreat B solution	100 ml
WB Solution	100 ml
10X Wash solution	100 ml
HRP substrate	2 x 30 ml
Nitrocellulose membrane	10 Sheets
Protocol	1
Manual	TM0211

Document

TECHNICAL MANUAL: 1694_20060721033243.PDF (PDF)
Document-COA: 9820_20090524211855.PDF (PDF)
Document-COA: 10497_20090819035443.PDF (PDF)
Document-COA: 11450_20091202031528.PDF (PDF)
Document-MSDS: 11913_20100223194255.PDF (PDF)
Document-COA: 11964_20100228200343.PDF (PDF)
Document-COA: 12684_20100427205305.PDF (PDF)
Document-COA: 14235_20111114035413.PDF (PDF)
Document-COA: 14236_20111114035414.PDF (PDF)
Document-COA: 14237_20111114035414.PDF (PDF)
Document-COA: 14238_20111114035414.PDF (PDF)

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