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 Name
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L00223-10
 High Affinity Ni-Charged Resin
10 ml
$80
L00223-25
 High Affinity Ni-Charged Resin
25 ml
$168
L00223-500
 High Affinity Ni-Charged Resin
500 ml
$2480

High Affinity Ni-Charged Resin


Description:
GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 ml lab packs.

Total Volume:
  • 10 ml settled resin (20 ml 50% slurry)
  • 25 ml settled resin (50 ml 50% slurry)
  • 500 ml settled resin (1000 ml 50% slurry)

Key Features:
  • High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/ml (CV).
  • Simple purification procedure under both native and denaturing conditions.
  • Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
  • Resilient: If properly regenerated, the resin can be reused multiple times.

Applications:
1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

Storage:
Store the product between 2°C and 8°C.

Note:
For ambient pressure use only.


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