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You are here: Products » Cellular Analysis » Apoptosis Detection Kits » TUNEL Detection Kits » Kit : TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)
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Cat. No.
 Name
Size   
Price
 Selected
L00297-100
 TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)
100 Assays
$598
L00297-20
 TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)
20 Assays
$238
L00297-50
 TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)
50 Assays
$421

TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)


Description

The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections (Biotin labeled POD ) (Cat. No. L00297) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K, DAB-Solution and DNase I.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
  • High Veracity: The kit contains positive control reagent.

    Technical Manual


    Components
    Equilibration Buffer 5.0 ml
    Biotin-11-dUTP 100 μl
    TdT 400 μl (for 100 assays)
    50× Proteinase K (1 mg/ml) 200 μl (for 100 assays)
    Streptavidin-HRP 50 μl (for 100 assays)
    DAB-A Solution 600μL (for 100 assays)
    DAB-B Solution 600μL (for 100 assays)
    DAB-C Solution 600μL (for 100 assays)


    Document


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