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Cat. No.
 Name
Size   
Price
 Selected
L00300-100
 TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)
100 Assays
$598
L00300-20
 TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)
20 Assays
$254
L00300-50
 TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)
50 Assays
$445

TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)

Description

The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections (FITC-labeled POD) (Cat. No. L00300) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, fluorescein-labeled nucleotides bind with the DNA 3'-OH ends using natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). The fluorescence could be observed by fluorescence microscope. And then the compound of anti-fluorescein antibody conjugated Peroxidase (POD) is bound to these fluorescein labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K, DAB-Solution and DNase I.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by fluorescence microscope and light microscope.
  • High Veracity: The kit contains positive control reagent.

    Technical Manual


    Components
    Equilibration Buffer 5.0 ml (for 100 assays)
    FITC-12-dUTP 100 μl (for 100 assays)
    TdT 400 μl (for 100 assays)
    50X Proteinase K （1 mg/ml） 200 μl (for 100 assays)
    POD-conjugated Anti-FITC antibody 1000 µl (for 100 assays)
    DAB-A Solution 600μL (for 100 assays)
    DAB-B Solution 600μL (for 100 assays)
    DAB-C Solution 600μL (for 100 assays)
    DNase I (50 U/µl) 1ml (for 100 assays)


    Document


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