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L00304
 Caspase-9 Colorimetric Assay Kit
1 kit
$275

Caspase-9 Colorimetric Assay Kit

Description:

The Caspase-9 Colorimetric Assay Kit (Cat. No. L00304) provides a simple and convenient means of assaying the activity of caspase-9. The activation of ICE-family proteases and caspases initiates apoptosis in mammalian cells. The assay for caspase-9 is based on spectrophotometric detection of the chromophore pnitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. By comparing the absorbance of pNA from an apoptotic sample to an uninduced control, the relative increase of caspase-9 activity can be determined.
Key features:

  • Simplified Procedure: The components in this kit are provided ready-to-use.
  • Supreme Reliability: The kit produces highly reproducible results.

    Technical Manual


    Components
    Lysis Buffer 15.0 ml
    2X Reaction Buffer 5.0 ml
    Substrate 500 ul, store away from light
    DTT 150 ul


    Document


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