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Cell Apoptosis PI Detection KitDiscriptionFluorescent labeling is fundamental to apoptosis research. It can be used to assay the morphological and biochemical changes involved in apoptosis in normal, apoptotic, and dead cells. GenScript's Cell Apoptosis PI Detection Kit (Cat.No.L00311) provides a rapid and convenient assay for apoptosis based upon fluorescent detection. The kit includes ready-to-use propidium iodide (PI), a DNA-specific red-fluorescence dye (excitation/emission maxima ≈535/617 nm). Propidium iodide is only permeant to dead cells. Under fluorescent microscopy, normal cells are not stained, while dead cells show strong red fluorescence. The intensity of the fluorescence of apoptotic cells is between dead cells and normal cells. Under flow cytometry, DNA in the cells is degraded by endogenous nuclease activity and diffused out of cells as part of apoptosis. A highly definable sub-G1 peak occurs and is easily quantified by PI. Cells can then be sorted and further analyzed by microscopy to verify the presence of apoptotic cells. The change in DNA of the apoptotic cells is then assayed by flow cytometry. Key features:
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