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GPCR Membrane Preparations

G-protein independent binding assays are preferred methods in assaying GPCR activation especially in primary screening. To meet the demand of membrane preparation, GenScript takes advantage of in-house GPCR stable cell lines validated by functional assays to offer GPCR membrane preparation products. All the membrane preparations have been validated by radioligand binding assay and/or Eu-GTP binding assay.

GenScript also offers custom large-scale membrane preparation production services utilizing the stable cell line from GenScript or client. Tailored to clients' specifications, [35S]GTPgammaS assay is available as an alternative QC method.

Key Features:

  • Consistent quality: All membrane preparations are from stable cell lines.
  • Receptor yield: High GPCR expression stable cell lines are used.
  • Quality control: Every batch of membrane preparations is validated by radioligand binding assay and/or Eu-GTP binding assay.
  • Large-scale production: 1010 cells are available for membrane production within one month.

Examples:

Saturation Binding for ADRA1A
Fig A. 10 μg of membranes prepared from CHO cells stably expressing ADRA1A receptors were incubated with indicated concentrations of [3H]Prazosin in the absence (total binding) or presence of 1000-fold access unlabeled Prazosin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.
 
Saturation Binding for ADRA1B
Fig B. 10 μg of membranes prepared from CHO cells stably expressing ADRA1B receptors were incubated with indicated concentrations of [3H]Prazosin in the absence (total binding) or presence of 1000-fold access unlabeled Prazosin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

Available Membrane Preparations:

    To order membrane preparation service with customer provided stable cell lines

Sample Requiremnet
If you want to produce membranes utilizing your stable cell line, please submit 2 vials cells (3 x 106 cells/vial).
Timeline:
< 10 mg: 4 weeks;
< 50 mg: 5 weeks.
> 50 mg: Please inquire.
Delivery:
Standard aliquote: 1 mg/vial. For custom aliquot, Please inquire.
QC Standard:
Radioligand binding assay and/or Eu-GTP binding assay. Bmax and Kd value comparison with data from literature.
Every batch of membrane preparation will be quality controlled. The QC report will be delivered along with the products.

Cat. No. Service
Price (USD)
SC1486
Custom Membrane Production Service
Quotation

    To order membrane preparation service with GenScript’s in-house GPCR stable cell lines

Cat. No. Name Family Receptor
Size
Price (USD)
 Selected
CHO-K1/ADRA1A Adrenoceptors ADRA1A
1 vial
$320.00
CHO-K1/ADRA1B Adrenoceptors ADRA1B
1 vial
$320.00
293/5-HT2C Serotonin 5-HT2C
1 vial
$320.00
CHO-K1/M1 Acetylcholine (muscarinic) M1
1 vial
$320.00
CHO-K1/M3 Acetylcholine (muscarinic) M3
1 vial
$320.00
CHO-K1/M5 Acetylcholine (muscarinic) M5
1 vial
$320.00
CHO-K1/ADRB1/Gα15 Adrenoceptors ADRB1
1 vial
$320.00
CHO-K1/BB2 Bombesin BB2
1 vial
$320.00
CHO-K1/BB3 Bombesin BB3
1 vial
$320.00
CHO-K1/B2/Gα15 Bradykinin B2
1 vial
$320.00
CHO-K1/CMKLR1/Gα15 Chemoattractant CMKLR1
1 vial
$320.00
CHO-K1/CCKA/Gα15 Cholecystokinin CCKA
1 vial
$320.00
CHO-K1/CCKB Cholecystokinin CCKB
1 vial
$320.00
CHO-K1/CRF2/Gα15 Corticotropin Releasing Factor CRF2
1 vial
$320.00
CHO-K1/D2/Gα15 Dopamine D2
1 vial
$320.00
CHO-K1/D2s/Gα15 Dopamine D2s
1 vial
$320.00
CHO-K1/EDNRA Endothelin EDNRA
1 vial
$320.00
CHO-K1/EDNRB Endothelin EDNRB
1 vial
$320.00
CHO-K1/GPR40/Gα15 Free Fatty Acid FFA1/GPR40
1 vial
$320.00
CHO-K1/GAL1/Gα15 Galanin GAL1
1 vial
$320.00
CHO-K1/GAL2 Galanin GAL2
1 vial
$320.00
CHO-K1/GHSR Ghrelin GHSR
1 vial
$320.00
293/H1 Histamine H1
1 vial
$320.00
CHO-K1/H4/Gα15 Histamine H4
1 vial
$320.00
CHO-K1/EDG3/Gα15 Lysophospholipid EDG3
1 vial
$320.00
CHO-K1/EDG5/Gα15 Lysophospholipid EDG5
1 vial
$320.00
CHO-K1/EDG8/Gα15 Lysophospholipid EDG8
1 vial
$320.00
293/MCH1 Melanin-concentrating Hormone MCH1
1 vial
$320.00
CHO-K1/MCH2 Melanin-concentrating Hormone MCH2
1 vial
$320.00
CHO-K1/MT2/Gα15 Melatonin MT2
1 vial
$320.00
CHO-K1/NMU1 Neuromedin U NMU1
1 vial
$320.00
CHO-K1/NTS1 Neurotensin NTS1
1 vial
$320.00
CHO-K1/FPRL1/Gα15 N-Formyl Peptide FPRL1
1 vial
$320.00
CHO-K1/OPRK1/Gα15 Opioid OPRK1
1 vial
$320.00
CHO-K1/OPRM1/Gα15 Opioid OPRM1
1 vial
$320.00
CHO-K1/OX1 Orexin OX1
1 vial
$320.00
CHO-K1/OXTR Oxytocin OT/OXTR
1 vial
$320.00
CHO-K1/PTAFR Platelet Activating Factor PTAFR
1 vial
$320.00
293/FP/Gα15 Prostanoid FP
1 vial
$320.00
293/PTGER1 Prostanoid PTGER1
1 vial
$320.00
CHO-K1/PAR4 Proteinase-activated Receptor PAR4
1 vial
$320.00
1321N1/P2Y4 Purinergic P2Y4
1 vial
$320.00
1321N1/P2Y11 Purinergic P2Y11
1 vial
$320.00
CHO-K1/5-HT2A Serotonin 5-HT2A
1 vial
$320.00
CHO-K1/5-HT2B Serotonin 5-HT2B
1 vial
$320.00
CHO-K1/SST3/Gα15 Somatostatin SST3
1 vial
$320.00
CHO-K1/SST5/Gα15 Somatostatin SST5
1 vial
$320.00
CHO-K1/NK1 Tachykinin NK1/SPR
1 vial
$320.00
CHO-K1/NK2 Tachykinin NK2
1 vial
$320.00
CHO-K1/NK3 Tachykinin NK3
1 vial
$320.00
CHO-K1/TRH1 Thyrtropin-releasing Hormone TRH1
1 vial
$320.00
CHO-K1/V1A Vasopressin V1A
1 vial
$320.00
CHO-K1/V1B Vasopressin V1B
1 vial
$320.00
For bulk order, please inquire.
Note: 1 vial contains 1 mg membrane protein.

Related Custom Products and Services:


Email: bioassay@genscript.com
Phone: 1-877-436-7274 (Toll-Free), 1-732-885-9188
Fax: 1-732-210-0262, 1-732-885-5878