| Product Name |
T-24 |
Species |
Human |
| Documents |
|---|
| Figures |
|---|
| Reference |
|---|
Stabenow D,, et al. Cdk2 activity is dispensable for triggering replicon initiation after transient hypoxia in T24 cells. FEBS J. Nov 2005; 272(21): 5623-5634.
van Betteraey-Nikoleit M, et al. Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells. Eur. J Biochem. Oct 2003; 270(19): 3880-3890.
van Bokhoven A, et al. TSU-Pr1 and JCA-1 cells are derivatives of T24 bladder carcinoma cells and are not of prostatic origin. Cancer Res. Sep 2001; 61(17): 6340-6344.
|
|
Tissue |
Genito-Urinary |
Description |
Human urinary carcinoma. T24 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. |
Concentration |
100 μg/100 μl. Whole lysate in 1 X SDS 5% b-mercaptoethanol. |
Note |
Cell line: T24 (Transitional-cell human bladder carcinoma)
Growth media: DMEM & 10% FBS (Fetal bovine serum). |
Storage |
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
Purity |
Whole Cell Lysate.
|
Application |
T24 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane isrecommended for mini gel. |
|
