| Product Name |
Raji |
Species |
Human |
| Documents |
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 Document-EXAMPLE: 1652_20060711033644.JPG (JPG) |
| Figures |
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| Reference |
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Jurisic V, et al. Effect of TNF-alpha on Raji cells at different cellular levels estimated by various methods. Ann. Hematol. Feb 2006; 85(2): 86-94.
Chen WH, et al. Deguelin inhibits expression of IkappaBalpha protein in Raji and U937 cells. Acta. Pharmacol Sin. Apr 2006; 27(4): 485-90.
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Tissue |
B cell lymphoma |
Description |
Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate |
Concentration |
Concentration: 100 μg/100 μl.
Storage buffer: Whole lysate in 1 X SDS 5% b-mercaptoethanol.
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Note |
Cell line: Raji (Human Burkitt's lymphoma).
Growth media: RPMI & 10% NCS (Newborn calf serum).
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Recommended loading volume |
Raji cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane isrecommended for mini gel. |
Storage |
Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Background |
Raji cell line is the first continuous human hematopoietic cell line. It has become an indispensable tool in haematological research and leukaemia and lymphoma studies. Raji cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
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Document-EXAMPLE: 1652_20060711033644.JPG (JPG)