| Product Name |
Multiple Tag Cell Lysate |
Species |
E. coli |
|
Description |
Multiple Tag Cell Lysate is whole E. coli cell lysate comprised of about 30% specially designed fusion protein, which act as a positive control for Western blot or Dot blot. The fusion protein is a 52 kD protein containing 16 commonly used tags, which are, in order, Trx, T7, HSV, C-Myc, SVS-g, Glu-Glu, V5, E tag, DYKDDDDK, S-tag, HA, KT3, E2, AU1, AU5, and His tag. |
Concentration |
200 µg of whole E. coli cell lysate (lyophilized) |
Recommended loading volume |
Add 200 µl of ddH2O to make the final concentration of 1 µg/µl in 1X SDS Loading Buffer. 0.02 – 0.5 µl of the lysate is recommended for each lane of a minigel. For accurate loading, dilute with 1X SDS Loading Buffer. |
Storage |
Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below. |
Application |
Positive control for Western blot and Dot blot. |
|
Document-PRODUCTINFO: 2382_20060922114657.PDF (PDF)