| Full Name |
Myc (Phospho-Thr358) PAb Blocking Peptide |
| Description |
The synthesized phosphopeptide was derived from human Myc around the phosphorylation site of threonine 358 (R-R-TP-H-N). |
|
| Storage |
Store at -20°C |
| Concentration |
100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA, 5%DMF and 5% glycerol. |
| Quality Control |
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. |
| Applications |
For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot. For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides. |
| Specificity |
The peptide specifically blocks the signal of Rabbit Anti Myc (Phospho-Thr358) completely in western blotting and immunohistochemistry.
|
*For Non-Clinical Research Use Only*
MSDS: 20081008023758 (PDF)
