| Full Name |
Myc (Phospho-Ser373) PAb Blocking Peptide |
| Description |
The synthesized non-phosphopeptide was derived from human Myc around the phosphorylation site of serine 373.
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| Storage |
Store at -20°C |
| Notes |
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| Concentration |
100ug/10 mM phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM N |
| Quality Control |
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. |
| Applications |
For Western blotting: add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the blot.
For immunohistochemistry: add 2 µl of antibody and 2 µl of blocking peptide to 0.096 ml of buffer, and incubate at 4°C over night or at room temperature for 2 hours before allowing to react with the slides. |
| Specificity |
The peptide specifically blocks the signal of Rabbit Anti Myc (Phospho-Ser373) completely in Western blotting and immunohistochemistry.
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Document-MSDS: 6258_20081008025720.PDF (PDF)
