You are here: Products » Recombinant Proteins » Cytokines and Growth Factors » Epidermal Growth Factor, human

Cat. No.	Name	Size	Price
Z00333-1	Epidermal Growth Factor (EGF), human	1 mg	$225
Z00333-10	Epidermal Growth Factor (EGF), human	10 μg	$35
Z00333-50	Epidermal Growth Factor (EGF), human	50 μg	$120

Epidermal Growth Factor (EGF), human

Full Name

Epidermal Growth Factor (EGF), Human

Abbreviated Name-1

rHuEGF; Epidermal Growth Factor

Documents
Document-EXAMPLE: 2258_20060807005513.JPG (JPG)
Document-COA: 13674_20120206003941.PDF (PDF)
Document-MSDS: 15082_20111227195216.PDF (PDF)
Figures
Reference
Katagiri S, Takahashi Y. Potential relationship between normalization of endometrial epidermal growth factor profile and restoration of fertility in repeat breeder cows. Anim. Reprod. Sci. Sep 2006; 95(1-2): 54-66. Endo K, et al. Evaluation of the epidermal growth factor receptor gene mutation and copy number in non-small cell lung cancer with gefitinib therapy. Oncol. Rep. Sep 2006; 16(3): 533-541.

Description

Human Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues.

Source

E. coli

M.W.

6.0 kDa+/-10% determined by reduced SDS-PAGE

UV Scan

The maximal absorption wave is 275+/-3 nm.

Purity

Greater than 95% as determined by
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

Endotoxin Level

Less than 0.1ng/μg (1 IEU/μg) determined by LAL test.

Specific Activity

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×10⁵ IU/ mg.

Storage

Lyophilized Recombinant Human Epidermal Growth Factor (EGF) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution Recombinant Human Epidermal Growth Factor (EGF) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.

Formulation

The protein was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.

Reconstitution

It is recommended to reconstitute the lyophilized rHuEGF in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Quantitation

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis, using a calibrated solution of EGF as a reference standard.

Sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Sequence analysis

The sequence of the first fifteen N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp-Gly-Tyr-Cys-Leu.

Note

;