Hercules Endonuclease

Full Name

Abbreviated Name-1

Hercules Endonuclease;

Documents
Document-MSDS: 7556_20081030013139.PDF (PDF)
Figures
Reference

Description

Hercules Endonuclease is a engineered endonuclease from Serratia marcescens. The endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2-5 bases in length. Hercules Endonuclease is compatible with Easy BacLysis Protein Extraction Solution.

Source

E. coli

Purity

Purity > 99% as determined by SDS-PAGE

Specific Activity

One unit of Hercules Endonuclease is defined as the amount of enzyme that causes an A₂₆₀ change of 1.0 in 30 minutes at 37°C, which corresponds to complete digestion of 37 μg of DNA.

Storage

Stable at 4°C for several weeks, for long-term storage, store it at -20°C after receiving. No obvious decrease of enzyme activity can be found after an incubation at 37°C for a week.

Concentration

200 U/μl in 10 mM Tris-HCl, pH8.0, 2 mM MgCl₂, 20 mM NaCl and 50% glycerol

Applications

Viscosity reduction in protein extracts

Sample preparation for 2D gel electrophoresis

Removal of nucleic acid contaminants from recombinant protein preparations

Note

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