| Full Name |
Interleukin-8 (IL-8) (3-79), Human
|
Abbreviated Name-1 |
rHu IL-8 (3-79); |
|
Description |
Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 (IL-8) levels are associated with the onset of a variety of disease states.
Human Interleukin-8 (IL-8) (3-79) , produced in E. coli, is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8,904 Da.
|
Source |
E. coli |
M.W. |
9,000 Da+/-10%, determined by reduced SDS-PAGE |
Purity |
The purity of GenScript rHuIL-8 is greater than 95.0%, as determined by the following methods:
(a) RP-HPLC analysis
(b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis |
Endotoxin Level |
Less than 0.1ng/µg (1 IEU/µg) determined by LAL test. |
Storage |
Although lyophilized Recombinant Human Interleukin-8 (IL-8)/77 can remain stable at room temperature for three weeks, it is best stored desiccated below -18°C. After reconstitution, Recombinant Human Interleukin-8 (IL-8)/77 may be stored at 4°C for up to seven days and below -18°C for longer periods. For long-term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Please avoid freeze-thaw cycles. |
Formulation |
The protein was lyophilized after extensive dialysis against PBS, pH7.4. |
Reconstitution |
It is recommended that lyophilized GenScript rHuIL-8 /77 be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Specificity |
GenScript rHuIL-8 /77 is fully biologically active when compared to standard. The ED50, as determined by its chemotaxis of hCXCR-2 transfected mouse BaF/3 cells, is less than 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/mg. |
Sequence |
The sequence of the first five N-terminal amino acids has been found to be Ala-Val-Leu-Pro-Arg. |
Note |
; |
Document-MSDS: 15100_20111227202529.PDF (PDF)