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Other vector |
siRNA Expression Vector |
siRNA positive control |
siRNA cassette control |
siRNA negative control
pRNA-Luc/NeoDescriptionThe pRNA-Luc/Neo includes a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The purpose of this reporter vector is to screen for efficient siRNA for the target gene using Luc activity as a reporter gene. The principle is that when a siRNA silence the target gene by degrading mRNA, the Luc will not be expressed either, because the mRNA for both the gene and Luc as a whole molecule is degraded. The assay of this genetic reporter is rapid, sensitive and quantitative. StorageStore at -20°CDetailed MapF1 ori: 3250 - 3678SV40 ori & promoter: 3683 - 3851 CMV Promoter: 251 - 818 Neomycin: 4088 - 4882 pUC ori: 5569 - 6240 Ampicillin: 6384 - 7244 Vector Sequence and Restriction Enzyme Map Example
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