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Other vector | siRNA Expression Vector | siRNA positive control | siRNA cassette control | siRNA negative control
Cat. No.
 Name
Size   
Price
 Selected
SD1216
 PRNAT-H1.1/shuttle
10 μg
$350

pRNAT-H1.1/Shuttle

Description
pRNAT-H1.1/Shuttle is an adenoviral siRNA shuttle vector. It is compatible with the Clontech Adeno-XTM Expression System 1. It carries a GFP marker (coral GFP, cGFP) under CMV promoter control. This marker can be used to track the transfection efficiency. It uses an H1 promoter for siRNA expression.



Storage
Store at -20°C


Download Protocol
tm0165


Forward Sequencing Primer
DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 6164 - 6187
H1 Promoter: 6064 - 6163
CMV Promoter: 37 - 624
cGFP: 641 - 1357
SV40 Promoter: 2085 - 2430
Neomycin: 2471 - 3265
pUC ori: 3979 - 4619
Ampicillin: 4767 - 5627

Vector Sequence and Restriction Enzyme Map

Limited Use Label License

The use of CMV promoter is covered under U. S. Patent No. 5,168,062 and 5,385,839 owned and licensed by the University of Iowa Research Foundation and is sold for research use only. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation (UIRF), 214 Technology Innovation Center, Iowa City, Iowa 52242. For further information, please contact the Associate Director of UIRF, at 319-335-4546.

Document

  • Document-PRODUCTINFO: 1133_20051023224312.PDF (PDF)
  • TECHNICAL MANUAL: 339_20050517003536.PDF (PDF)

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