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Other vector | siRNA Expression Vector | siRNA positive control | siRNA cassette control | siRNA negative control
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SD1233
 pRNA-CMV3.1-Puro
10 μg
$225

pRNA-CMV3.1/Puro


Description
Human cytomegalovirus (CMV) promoter is one of the strongest promoters described. Based on RNA polymerase II system, CMV promoter drives higher-level constitutive expression of genes in a variety of mammalian cell lines, compared with RNA polymerase III based promoters such as U6 and H1. In this vector, CMV promoter drives the expression of siRNA, and SV40 promoter drives the expression of the resistance gene. siRNA cassettes can be easily inserted into the vectors between BamH I and Hind III sites. siRNA sequence can be confirmed by DNA sequencing using Reverse Sequencing Primer DA0012 (5’-TAGAAGGCACAGTCGAGG-3’)




Storage
Store at -20°C


Download Protocol
tm0172


Forward Sequencing Primer
DA0023: pRNA-CMV Forward (GTACGGTGGGAGGTCTATAT)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Puromycin: 1598 - 2197
Polylinker: 584 - 651
CMV Promoter: 1 - 583
SV40 Promoter: 1267 - 1598
pUC ori: 2896 - 3536
Ampicillin: 3684 - 4544

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1149_20051023234759.PDF (PDF)
  • TECHNICAL MANUAL: 412_20050718094354.PDF (PDF)

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