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Other vector | siRNA Expression Vector | siRNA positive control | siRNA cassette control | siRNA negative control
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 Name
Size   
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 Selected
SD1263
 pRNAT-CMV3.1-Puro
10 μg
$350

pRNAT-CMV3.1/Puro


Description
The human cytomegalovirus (CMV) promoter is one of the strongest promoters described. Based on the RNA polymerase II system, the CMV promoter drives higher-level constitutive expression of genes in a greater variety of mammalian cell lines than RNA polymerase III-based promoters, such as U6 and H1. A CMV promoter drives the expression of siRNA, and an SV40 promoter drives the expression of the resistance gene. siRNA cassettes can be easily inserted into the vectors between BamH I and Afl II sites. The siRNA sequence can be confirmed by DNA sequencing using reverse sequencing primer DA0012 (5’-TAGAAGGCACAGTCGAGG-3’). This vector also carries cGFP (coral GFP) for convenient tracking of transfection efficiency.




Storage
Store at -20°C


Download Protocol
tm0172


Forward Sequencing Primer
DA0023: pRNA-CMV Forward (GTACGGTGGGAGGTCTATAT)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Puromycin: 2911 - 3510
Polylinker: 584 - 651
CMV Promoter: 1 - 583
cGFP: 1598 - 2317
SV40 Promoter: 1267 - 1598
pUC ori: 4210 - 4850
Ampicillin: 4998 - 5858

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1167_20051024022606.PDF (PDF)
  • TECHNICAL MANUAL: 412_20050718094354.PDF (PDF)

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