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Other vector | siRNA Expression Vector | siRNA positive control | siRNA cassette control | siRNA negative control
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SD1553
 pRNA-H1.3/Neo/siFluc
10 μg
$225

pRNA-H1.3/Neo/siFLuc


Description
pRNA-H1.3/Neo/siFLuc is a GenScript siRNA expression vector. It is designed for mammalian transfection. It uses an enhanced H1 promoter (enhanced by a CMV enhancer just before H1 promoter) for siRNA expression. This vector contains siRNA construct for Firefly luciferase, and can be used as a positive control. It can also be used as a siRNA vector using BamH I and Hind III for siRNA insertion.




Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0024: pRNA-H1.3 Forward (GACGTCAATGGGAGTTTGTT)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

CMV IE: 4559 - 5042
siFluc: 5148 - 5205
Polylinker: 5142 - 5211
H1 Promoter: 5049 - 5143
SV40 Promoter: 647 - 992
Neomycin: 1033 - 1827
pUC ori: 2541 - 3181
Ampicillin: 3329 - 4189

Vector Sequence and Restriction Enzyme Map



Document

  • Document-DATASHEET: 1191_20051024030347.PDF (PDF)

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