24 hour
You are here: Products » PCR and Cloning » siRNA Expression Vector » siRNA Vector : pRNAT-H1.3/Hygro
GenScript Share
Other vector | siRNA Expression Vector | siRNA positive control | siRNA cassette control | siRNA negative control
Cat. No.
 Name
Size   
Price
 Selected
SD1563
 pRNAT-H1.3/Hygro
10 μg
$350

pRNAT-H1.3/Hygro

Description
pRNAT-H1.3/Hygro is a GenScript siRNA expression vector. It is designed for mammalian transfection. It carries a hygromycin resistance gene as a selectable marker, which can be used for establishing stable cell lines. It also has a coral GFP marker (cGFP) under CMV promoter control, which can be used to track the transfection efficiency. It uses a CMV-enhanced H1 promoter for siRNA expression. The siRNA insert can be cloned between BamH I and Hind III sites.




Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0032: SD1563 Forward (CTGGGAAATCACCATAAACG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

CMV IE: 7 - 490
Polylinker: 590 - 659
H1 Promoter: 497 - 591
CMV Promoter: 698 - 1285
cGFP: 1302 - 2021
SV40 Promoter: 2710 - 3055
Hygromycin: 3078 - 4103
pUC ori: 4773 - 5413
Ampicillin: 5561 - 6421

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1197_20051024032633.PDF (PDF)

    • Related Products
    Cat. No.
    		 Name
    Size   
    Price
    		 Selected
    A00185-200
    		 cGFP-tag Antibody, mAb, Mouse
    200 μg(5x40μg)
    $225
    A00185-40
    		 cGFP-tag Antibody, mAb, Mouse
    40 μg
    $50