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*This product has been discontinued! *

QuickClean 5M Gel Extraction Kit


Description:
GenScript QuickClean 5M Gel Extraction Kit offers fast DNA extraction and purification from both standard and low-melt agarose gels. Up to 20 μg of double- or single-stranded DNA can be recovered from a solubilized gel slice in just five minutes. DNA molecules of anywhere between 70 and 10,000 base pairs absorb to the Genprep Column and contaminants and inhibitors such as agarose, dyes, and salts are washed away. Eluted in a small volume of low-salt buffer, the purified DNA is immediately ready for downstream applications such as PCR, transformation, restriction enzyme digestion, cloning, sequencing, in vitro translation, and transfection.

Key Features:

  • Easy to perform: Simple and rapid procedure to purify DNA in 5 min from solubilized gel slice.
  • Minimal preparation time: QuickClean's simple and rapid procedure purifies DNA in 5 - 10 minutes.
  • High capacity: Each column has a capacity of 20 µg plasmid DNA.
  • High purity: The kit completely removes contaminants and inhibitors.
  • Reproducible yields: Recovery is typically between 90% and 95% pure plasmid DNA, reproducible every time.
Applications:

The QuickClean 5M Gel Extraction Kit enables fast, high-quality extraction and purification of DNA from agarose gels. This DNA is immediately ready for further downstream applications such as the following:
  • PCR and cloning
  • Restriction enzyme digestion
  • Transformation
  • Sequencing
  • in vitro translation
Examples:

Shown below is a comparison of the QuickClean 5M Gel Extraction Kit to an anonymous competitor.

Figure 1.The QuickClean 5M Gel Extraction Kit and a kit from Competitor A were to extract and purify DNA from agarose gels. Both kits were used in accordance with the protocols provided by the manufacturers. Two DNA purifications were performed using each kit. The same amount of DNA was loaded into four wells of the agarose gel used in this test. Lane 1 shows unpurified material.
Technical Manual:

Components:
Components  L00199 
Elution Buffer  25 ml 
Genprep Columns  250 
Protocol  1 
Wash Solution  55 ml 
2 ml Collection Tubes  250 
Binding Solution II  2 x 110 ml 

Document:

1
 Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
2
 PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
3
 Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
4
 Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.
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