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L00274
 Protein G MagBeads
2 ml
$125

Protein G MagBeads

Description:

GenScript Protein G MagBeads are ideal for small-scale antibody purification and immunoprecipitation of proteins, protein complexes or other antigens.

GenScript Protein G MagBeads are superparamagnetic beads of average 40 μm in diameter, covalently coated with recombinant Protein G. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains and also the sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.

Key Features:

  • Quick and convenient separation accomplished by magnetic forces
  • High binding capacity more than 10 mg goat IgG/ml settled beads
  • Extremely low nonspecific binding

    • Technical Manual


    Components
    Pretreat A-a solution 100 ml
    Pretreat A-b solution 100 ml
    Pretreat A-c solution 100 ml
    Pretreat A-d solution 100 ml
    Pretreat B 2 x 100 ml
    5-Slot Dot blot plate 1
    Protocol 1


    Document