GenScript Protein G MagBeads are ideal for small-scale antibody purification and immunoprecipitation of proteins, protein complexes or other antigens.
GenScript Protein G MagBeads are superparamagnetic beads of average 40 μm in diameter, covalently coated with recombinant Protein G. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains and also the sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.
Total Volume:0.5 ml settled Beads (2 ml 25% slurry)
Store at 4℃, do NOT freeze.
|Cat. No. L00274|