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L00290-100
 TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)
100 Assays
$598
L00290-20
 TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)
20 Assays
$246
L00290-50
 TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)
50 Assays
$437

TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)


Description

The TUNEL Universal Apoptosis Detection Kit (Biotin-labeled POD) (Cat. No. L00290) is one of GenScript’s newly introduced products. It is used for assaying adherent Cells, paraffin-embedded tissue sections and cryopreserved tissue sections. The Kit could detect the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase (TdT or rTdT). And then horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-Diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including Proteinase K, DAB-Solution and DNase I.
  • Enhanced Sensitivity: The kit can assay the cells in early period of apoptosis.
  • Enhanced Specificity: The kit can stain apoptosis cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The whole process takes about three hours.
  • Increased Convenience: The result can be observed by light microscope.
  • High Veracity: The kit contains positive control reagent.

    Technical Manual


    Components
    Equilibration Buffer 5 ml
    Biotin-11-dUTP 100 μl
    TdT 400 μl
    50X Proteinase K (1mg/ml) 200 μl (for 100 assays)
    Streptavidin-HRP 50 μl
    DAB-A Solution 600μL (for 100 assays)
    DAB-B Solution 600μL (for 100 assays)
    DAB-C Solution 600μL (for 100 assays)


    Document


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