Catalog Products » Caspase Colorimetric Assay Kits » Caspase-2 Colorimetric Assay Kit
*This product has been discontinued! *

Caspase-2 Colorimetric Assay Kit

Description:

The Caspase-2 Colorimetric Assay Kit (Cat. No. L00294) provides a simple and convenient means of assaying the activity of caspase-2. Activation of ICE-family proteases and caspases initiates apoptosis in mammalian cells. The assay for caspase-2 is based on spectrophotometric detection of the chromophore pnitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. By comparing the absorbance of pNA from an apoptotic sample to an uninduced control, the relative increase of caspase-2 activity can be determined.
Key features:

  • Simple and rapid: The components in this kit are provided ready-to-use.
  • Supreme Reliability: The kit produces highly reproducible results.

    Technical Manual
  • TECHNICAL MANUAL: 3925_20080219220959.pdf (pdf)


  • Components
    Lysis Buffer 15.0 ml
    2X Reaction Buffer 5.0 ml
    Caspase-2 Substrate 500 μl, store away from light
    DTT 150 μl (1.0 M)
    PMSF 200 μl (100 mM)


    Document


    1
    Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
    2
    Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
    3
    Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
    4
    Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
    5
    Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.