24 hour
You are here: Products » Cellular Analysis » Apoptosis Detection Kits » Caspase Colorimetric Assay Kits » Kit : Caspase-2 Colorimetric Assay Kit
GenScript Share
Cat. No.
 Name
Size   
Price
 Selected
L00294
 Caspase-2 Colorimetric Assay Kit
1 kit
$275

Caspase-2 Colorimetric Assay Kit

Description:

The Caspase-2 Colorimetric Assay Kit (Cat. No. L00294) provides a simple and convenient means of assaying the activity of caspase-2. Activation of ICE-family proteases and caspases initiates apoptosis in mammalian cells. The assay for caspase-2 is based on spectrophotometric detection of the chromophore pnitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. By comparing the absorbance of pNA from an apoptotic sample to an uninduced control, the relative increase of caspase-2 activity can be determined.
Key features:

  • Simple and rapid: The components in this kit are provided ready-to-use.
  • Supreme Reliability: The kit produces highly reproducible results.

    Technical Manual


    Components
    Lysis Buffer 15.0 ml
    2X Reaction Buffer 5.0 ml
    Substrate 500 ul, store away from light
    DTT 150 ul


    Document


    Related Products
    Cat. No.
    		 Name
    Size   
    Price
    		 Selected
    L00289
    		 Caspase-3 Colorimetric Assay Kit
    1 kit
    $275
    L00302
    		 Caspase-6 Colorimetric Assay Kit
    1 kit
    $275
    1
    		 Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
    2
    		 Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
    3
    		 Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
    4
    		 Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
    5
    		 Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.
    		•