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Cat. No.
 Name
Size   
Price
 Selected
L00296-100
 TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)
100 Assays
$598
L00296-20
 TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)
20 Assays
$238
L00296-50
 TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)
50 Assays
$421

TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)


Description

The TUNEL Apoptosis Detection Kit for Adherent Cells (Biotin-labeled POD)(Cat. No. L00296) is one of GenScript’s newly introduced products. It is used for detecting the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase (TdT or rTdT). And then horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide and 3,3’-Diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key Feature
  • Simplified Procedure: The kit contains ready-to-use reagents, including DAB-Solution and DNase I.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
  • High Veracity: The kit contains positive control reagent.

    Technical Manual


    Components
    Equilibration Buffer 5.0 ml
    Biotin-11-dUTP 100 μl
    TdT 400 μl
    Streptavidin-HRP 50 μl
    DAB-A Solution 600μL (for 100 assays)
    DAB-B Solution 600μL (for 100 assays)
    DAB-C Solution 600μL (for 100 assays)


    Document


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