Cellular Analysis
24 hour
You are here: Products » Cellular Analysis » Apoptosis Detection Kits » TUNEL Detection Kits » Kit : TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)
GenScript Share
Cat. No.
 Name
Size   
Price
 Selected
L00297
 TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)
100 Assays
$598.00
20 Assays
$238.00
50 Assays
$421.00

TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)


Description

The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections (Biotin labeled POD ) (Cat. No. L00297) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K, DAB-Solution and DNase I.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
  • High Veracity: The kit contains positive control reagent.
    Technical Manual:

    Components
    Components  L00297 
    DAB-A Solution  600μL (for 100 assays) 
    DAB-B Solution  600μL (for 100 assays) 
    DAB-C Solution  600μL (for 100 assays) 
    DNase I (50 U/µl)  1ml (for 100 assays) 
    DNase I buffer  1ml (for 100 assays) 
    Equilibration Buffer  5.0 ml (for 100 assays) 
    Streptavidin-HRP  50 μl (for 100 assays) 
    TdT  400 μl (for 100 assays) 
    50× Proteinase K (1 mg/ml)  200 μl (for 100 assays) 
    Biotin-11-dUTP  100 μl (for 100 assays) 


    Document


    Related Products
    Cat. No.
    		 Name
    Size   
    Price
    		 Selected
    L00290
    		 TUNEL Universal Apoptosis Detection Kit (Biotin labeled POD)
    100 Assays
    $598.00
    20 Assays
    $246.00
    50 Assays
    $437.00
    L00296
    		 TUNEL Apoptosis Detection Kit (For Adherent Cells, Biotin-labeled POD)
    100 Assays
    $598.00
    20 Assays
    $238.00
    50 Assays
    $421.00
    L00298
    		 TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Sections, Biotin labeled POD)
    100 Assays
    $598.00
    L00299
    		 TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)
    100 Assays
    $598.00
    20 Assays
    $254.00
    50 Assays
    $445.00
    L00300
    		 TUNEL Apoptosis Detection Kit (For Paraffin-embedded Tissue Section,FITC-labeled POD)
    100 Assays
    $598.00
    20 Assays
    $254.00
    50 Assays
    $445.00
    L00301
    		 TUNEL Apoptosis Detection Kit (For Cryopreserved Tissue Section, FITC labeled POD)
    100 Assays
    $598.00
    20 Assays
    $254.00
    50 Assays
    $445.00
    1
    		 Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
    2
    		 Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
    3
    		 Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
    4
    		 Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
    5
    		 Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.
    		•