TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)
Description
The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections (Biotin labeled POD ) (Cat. No. L00297) is one of GenScript's newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3-diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Key features
Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
Enhanced Specificity: The kit can stain apoptotic cells.
Streamlined Process: The entire procedure takes about three hours.
Increased Convenience: The results can be observed by light microscope.
Technical Manual:
Components:
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Components
|
L00300 |
L00299 |
L00290 |
L00296 |
L00297 |
L00301 |
|---|
|
DAB
|
10 mg |
10 mg |
10 mg |
10 mg |
10 mg |
10 mg |
|---|
|
DNase I
|
1ml (50000U) |
|
1ml (50000U) |
|
|
|
|---|
|
DNase I buffer
|
1ml |
|
1ml |
|
|
|
|---|
|
Equilibration Buffer
|
5.0 ml |
5.0 ml |
5.0 ml |
5.0 ml |
5.0 ml |
5.0 ml |
|---|
|
FITC-12-dUTP
|
100 μl |
100 μl |
|
|
|
100 μl |
|---|
|
HRP-labeled Anti-FITC Antibody
|
1000 μl |
1000 μl |
|
|
|
1000 μl |
|---|
|
Streptavidin-HRP
|
|
|
50 μl |
50 μl |
50 μl |
|
|---|
|
TdT
|
400 μl |
400 μl |
400 μl |
400 μl |
400 μl |
400 μl |
|---|
|
50X Proteinase K
|
200 μl |
|
200 μl |
|
200 μl |
|
|---|
|
Anti-fluorescein Antibody
|
|
|
|
|
|
|
|---|
|
Biotin-11-dUTP
|
|
|
100 μl |
100 μl |
100 μl |
|
|---|
Document:
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1 |
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