TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)

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*This product is under development*

TUNEL Apoptosis Detection Kit (Adherent Cell, FITC-labeled POD)

Description

The TUNEL Apoptosis Detection Kit for Adherent Cells (FITC-labeled POD) (Cat. No. L00299) is one of GenScriptâs newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotin-labeled nucleotides bind with the DNA 3'-OH ends using natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Streptavidin-FITC is then used to bind with biotin-labeled nucleotides, therefore,the fluorescence could be observed by fluorescence microscope. And then the compound of anti-fluorescein antibody conjugated Peroxidase (POD) is bound to these fluorescein labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3â-diaminobenzidine (DAB)-Solution,a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.

Key features

Simplified Procedure: The kit contains ready-to-use reagents, including DAB-Solution, and DNase I.

Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.

Enhanced Specificity: The kit can stain apoptotic cells.

Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.

Streamlined Process: The entire procedure takes about three hours.

Increased Convenience: The results can be observed by fluorescence microscope and light microscope.

Technical Manual

Components

Equilibration Buffer	5.0 ml (for 100 Assays)
Streptavidin-FITC	500μL (for 100 Assays)
1 X DNase I buffer	1ml
Biotin-11-dUTP	100 μl (for 100 Assays)
TdT	400 μl (for 100 Assays)
POD-conjugated Anti-FITC antibody	1000 μl (for 100 Assays)
DAB-A Solution	600μL (for 100 Assays)
DAB-B Solution	600μL (for 100 Assays)
DAB-C Solution	600μL (for 100 Assays)
DNase I (50 U/µl)	1ml
Labeling Buffer	5.0 ml (for 100 Assays)

Document

TECHNICAL MANUAL: L00299.pdf (pdf)
Document-MSDS: L00299 MSDS.pdf (pdf)
Document-COA: C54801210_L00299_COA.pdf (PDF)
Document-COA: C54811210_L00299_COA.pdf (PDF)
Document-COA: C54861207_L00299_COA.pdf (PDF)
Document-COA: C55131211_L00299_COA.pdf (PDF)
Document-COA: C55801208_L00299_COA.pdf (PDF)
Document-COA: C55811208_L00299_COA.pdf (PDF)
Document-COA: C57971211_L00299_COA.pdf (PDF)


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