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High-Affinity Iodoacetyl Resin
Description:GenScript High-Affinity Iodoacetyl Resin is produced by covalently coupling a derivative of iodoacetic acid to 4% cross-linked agarose beads. It is designed for easy and fast immobilization of sulfhydryl-containing peptides, proteins and other ligands. The resin reacts specifically with free sulfhydryls to form stable thioether linkage. The resin is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification. 1 ml settled resin can be coupled with more than 1 mg sulfhydryl-containing peptides of 7 amino-acids.
The High-Affinity Antibody Purification Kit contains High-Affinity Iodoacetyl Resin and all the necessary buffers needed for antibody purification.
Total Volume:10 ml settled resin ( 20 ml 50% slurry)
SDS-PAGE analysis of anti-His-tag IgG purified using Iodoacetyl Resin from Genscript and X company.
A peptide containing His-tag was coupled to the resin from GenScript and X company, respectively.
The flow through and the elution fractions were analyzed.
Lane 1: Smart Broad-Range Protein Standard (GenScript, MM0906)
Lane 2: Flow through (GenScript)
Lane 3: Elution (GenScript)
Lane 4: Flow through (X company)
Lane 5: Elution (X company)