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L00403
High-Affinity Iodoacetyl Resin
10 ml
$150.00

High-Affinity Iodoacetyl Resin

Description:

High-Affinity Iodoacetyl Resin (L00403) is prepared by covalently coupling a derivative of iodoacetic acid to 4% cross-linked agarose beads. It can be used for immobilization of sulfhydryl-containing peptides, proteins and other ligands. The resin reacts specifically with free sulfhydryls to form stable thioether linkage and is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification. 1 ml resin immobilizes more than 1 mg sulfhydryl-containing peptides of 7 amino-acids.
Key Features:
  • High stability: The resin is stable at a broad pH range from 5 to 10.
  • High binding capacity: More than 8 mg Rabbit IgG/ml resin.
  • Easy to use: The kit provides all the necessary buffers.

  • Characteristics:

    Binding Capacity More than 8 mg Rabbit IgG/ml resin.
    Ligand The derivative of Iodoacetic acid
    pH Stability pH 5 -10
    Support Matrix4% cross-linked agarose, spherical beads
    Mean Particle Size90 μm
    Storage>2°C to 8°C (Do not freeze.)
    Shelf Life12 months

    Examples:


    SDS-PAGE analysis of anti-His-tag IgG purified using Iodoacetyl Resin from Genscript and X company.
    A peptide containing His-tag was coupled to the resin from GenScript and X company, respectively.
    The flow through and the elution fractions were analyzed.
    Lane 1: Smart Broad-Range Protein Standard (GenScript, MM0906)
    Lane 2: Flow through (GenScript)
    Lane 3: Elution (GenScript)
    Lane 4: Flow through (X company)
    Lane 5: Elution (X company)
    Technical Manual:

    Components:

    Document:

    1
    Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
    2
    Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
    3
    Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.