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High-Affinity Iodoacetyl ResinDescription:High-Affinity Iodoacetyl Resin (L00403) is prepared by covalently coupling a derivative of iodoacetic acid to 4% cross-linked agarose beads. It can be used for immobilization of sulfhydryl-containing peptides, proteins and other ligands. The resin reacts specifically with free sulfhydryls to form stable thioether linkage and is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification. 1 ml resin immobilizes more than 1 mg sulfhydryl-containing peptides of 7 amino-acids. Key Features:Characteristics:
Examples:SDS-PAGE analysis of anti-His-tag IgG purified using Iodoacetyl Resin from Genscript and X company. A peptide containing His-tag was coupled to the resin from GenScript and X company, respectively. The flow through and the elution fractions were analyzed. Lane 1: Smart Broad-Range Protein Standard (GenScript, MM0906) Lane 2: Flow through (GenScript) Lane 3: Elution (GenScript) Lane 4: Flow through (X company) Lane 5: Elution (X company) Technical Manual:
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