Protein Isolation and Purification       
24 hour
You are here: Products » Proteins, Expression, Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » High-Affinity Iodoacetyl Resin
GenScript Share
Cat. No.
 Name
Size   
Price
 Selected
L00403
10 ml
$150

High-Affinity Iodoacetyl Resin


Description:
GenScript High-Affinity Iodoacetyl Resin is produced by covalently coupling a derivative of iodoacetic acid to 4% cross-linked agarose beads. It is designed for easy and fast immobilization of sulfhydryl-containing peptides, proteins and other ligands. The resin reacts specifically with free sulfhydryls to form stable thioether linkage. The resin is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification. 1 ml settled resin can be coupled with more than 1 mg sulfhydryl-containing peptides of 7 amino-acids.

The High-Affinity Antibody Purification Kit contains High-Affinity Iodoacetyl Resin and all the necessary buffers needed for antibody purification.

Total Volume:
10 ml settled resin ( 20 ml 50% slurry)

Key Features:
  • High stability: The resin is stable at a broad pH range from 5 to 10.
  • High binding capacity: More than 3 mg Rabbit IgG/ml resin.
  • Easy to use: The kit provides all the necessary buffers.

    • Characteristics:
    Binding Capacity More than 3 mg Rabbit IgG/ml resin.
    Ligand The derivative of Iodoacetic acid
    pH Stability pH 5 -10
    Support Matrix4% cross-linked agarose, spherical beads
    Mean Particle Size90 μm
    Storage2°C to 8°C
    Shelf Life12 months


    Examples:


    SDS-PAGE analysis of anti-His-tag IgG purified using Iodoacetyl Resin from Genscript and X company.
    A peptide containing His-tag was coupled to the resin from GenScript and X company, respectively.
    The flow through and the elution fractions were analyzed.
    Lane 1: Smart Broad-Range Protein Standard (GenScript, MM0906)
    Lane 2: Flow through (GenScript)
    Lane 3: Elution (GenScript)
    Lane 4: Flow through (X company)
    Lane 5: Elution (X company)

    Technical Manual


    Components
    High-Affinity Iodoacetyl Resin 10 ml (20 ml of 50% slurry)


    Document