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L00404
 High-Affinity Antibody Purification Kit
1 kit
$139.00

High-Affinity Antibody Purification Kit


Description:
GenScript High-Affinity Iodoacetyl Resin is produced by covalently coupling a derivative of iodoacetic acid to 4% cross-linked agarose beads. It is designed for easy and fast immobilization of sulfhydryl-containing peptides, proteins and other ligands. The resin reacts specifically with free sulfhydryls to form stable thioether linkage. The resin is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification. 1 ml settled resin can be coupled with more than 1 mg sulfhydryl-containing peptides of 7 amino-acids.

The High-Affinity Antibody Purification Kit contains High-Affinity Iodoacetyl Resin and all the necessary buffers needed for antibody purification.

Key Features:
  • High stability: The resin is stable at a broad pH range from 5 to 10.
  • High binding capacity: 1 ml resin can bind more than 3 mg Rabbit IgG.
  • Easy to use: The kit provides all the necessary buffers.


    • Components:
    High-Affinity Iodoacetyl Resin 5 ml (10 ml of 50% slurry)
    Empty Column3
    5X Coupling Buffer30 ml
    Block Buffer (Solid)10 ml (10 ml of 1X Coupling Buffer to the solid)
    Storage Buffer20 ml
    5X Binding/Wash Buffer70 ml
    Elution Buffer70 ml
    Neutralizing Buffer15 ml

    Examples:


    SDS-PAGE analysis of anti-His-tag IgG purified using Iodoacetyl Resin from GenScript and X company.
    A peptide containing His-tag was coupled to the resin from GenScript and X company, respectively.
    The flow through and the elution fractions were analyzed.
    Lane 1: Smart Broad-Range Protein Standard (GenScript, MM0906)
    Lane 2: Flow through (GenScript)
    Lane 3: Elution (GenScript)
    Lane 4: Flow through (X company)
    Lane 5: Elution (X company)
    Technical Manual:

    Components
    Components  L00433 


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