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L00404
 High-Affinity Antibody Purification Kit
1 kit
$139.00

High-Affinity Antibody Purification Kit

Description:

The High-Affinity Antibody Purification Kit (L00404) contains High-Affinity Iodoacetyl Resin and all the necessary buffers for antibody purification.
Key Features:

  • High stability: The resin is stable at a broad pH range from 5 to 10.
  • High binding capacity: 1 ml resin can bind more than 8 mg Rabbit IgG.
  • Easy to use: The kit provides all the necessary buffers.

    • Characteristics:

    High-Affinity Iodoacetyl Resin 5 ml (10 ml of 50% slurry)
    Empty Column3
    5X Coupling Buffer30 ml
    Block Buffer (Solid)10 ml (10 ml of 1X Coupling Buffer to the solid)
    Storage Buffer20 ml
    5X Binding/Wash Buffer70 ml
    Elution Buffer70 ml
    Neutralizing Buffer15 ml

    Examples:


    SDS-PAGE analysis of anti-His-tag IgG purified using Iodoacetyl Resin from GenScript and X company.
    A peptide containing His-tag was coupled to the resin from GenScript and X company, respectively.
    The flow through and the elution fractions were analyzed.
    Lane 1: Smart Broad-Range Protein Standard (GenScript, MM0906)
    Lane 2: Flow through (GenScript)
    Lane 3: Elution (GenScript)
    Lane 4: Flow through (X company)
    Lane 5: Elution (X company)
    Technical Manual:

    Components:

    1
    		 Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
    2
    		 Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
    3
    		 Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.