ToxinEraser<sup>TM</sup> Advanced Endotoxin Removal Kit

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ToxinEraser^TM Advanced Endotoxin Removal Kit

Description

ToxinEraser^TM Advanced Endotoxin Removal Kit is based on an endotoxin binding protein (EBP) developed from the horseshoe crab (Limulus polyphemus). Removal of endotoxin is so important because it is the principal pathophysiological mediator by which bacteria can cause hypotension, organ failure, disseminated intravascular coagulation, and fatal shock in mammalian hosts. In tissue culture and animal experiments, these responses can produce misleading results, making data uninterpretable, incurring unnecessary loss of time and money, and inevitable dragging the entire project.

GenScript ToxinEraser^TM Advanced Endotoxin Removal Kit is an efficient endototoxin removal tool. The final endotoxin level can be reduced below 1 EU/mg with repeated use of ToxinEraser^TM Advanced Endotoxin Removal resin, of which the characteristics of the resin are listed in Table 1. The kit can be used for removal of endotoxin from proteins, peptides, antibodies, and even DNA samples.

Key Features

High Binding Capacity: 2, 000, 000 EU / ml (CV)
High Recovery Yield: >90% with minimized sample loss
Greater than 95% Removal Efficiency
Reduce endotoxin level <0.01 EU/ug without much loss of DNA
Reusable up to five times without much activity loss

Technical Manual

Components

ToxinEraser^TM Advanced Endotoxin Removal Resin with Flow-Speed Control	1.5 ml pre-packed column
Regeneration Buffer	125 ml
Equilibration Buffer	125 ml
Collection Tubes	1 pack (3 per pack)
Tips (1 ml)	2 packs (6 per pack)
Pre-coated microwell plate (8 wells x 12 strips)	5
Dilution solution	100 ml
HRP conjugated GST monoclonal antibody	55 ml
GST protein stock (1.0 mg/ml)	40 ul
20X Wash solution	55 ml
One-Component TMB Substrate	55 ml
Stop solution	55 ml
Kit Manual	1

Document

Document-MSDS: 11267_20091124003914.PDF (PDF)
Document-COA: 14246_20111114035421.PDF (PDF)

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ToxinEraserTM Advanced Endotoxin Removal Kit