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QuickClean II Plasmid Miniprep Kit

QuickClean II Plasmid Miniprep Kit provides a fast, simple, and cost-effective method for purification of up to 20 μg high-copy plasmid from 1–5 ml of overnight cultures of E. coli. The DNA isolated by this Kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-Time PCR and other downstream experiments.

Key Features
The extracted DNA is immediately ready for routine molecular biology laboratory applications.
  • Fast Process: Complete 24 preps within 24 minutes.
  • High Purity: >90% removal of contaminants and inhibitors.
  • High Capacity: up to 20 μg DNA
  • High Reproducibility Yields:Up to 99 % recovery of plasmid DNA.

The extracted DNA is immediately ready for further downstream applications such as the following:
  • PCR and cloning
  • Restriction enzyme digestion
  • Transformation
  • High-quality sequencing
  • In vitro translation

Figure 1. Plasmid DNA preparation from E. coli bearing high copy number plasmid.
The QuickClean II Plasmid Miniprep Kit is here compared to a commercially available kit (Competitor A) for rapid plasmid purification from E. coli bearing high copy number plasmid. Both kits are used in compliance with the protocols provided by the manufacturers. The results are shown in Figure 1.

M1: DL15,000 DNA Marker (Takara)
Lane 1-3: Plasmid purified by GenScript kit
Lane 4-6: Plasmid purified by Competitor A

Figure 2. Enzymatic reactions analysis

M1: DL2,000TM DNA Marker (Takara)
M2: DL15,000 DNA Marker (Takara)
Lane1: Control
Lane 2 to 9: Hind III, Hac III, Dra I, Kpn I, BamH I, Hinf I, Xho I, EcoR I

Technical Manual
  • TECHNICAL MANUAL: L00420_V1protocol.pdf (pdf)

  • Components
    Resuspension Buffer 25 ml
    Lysis Buffer 25 ml
    Neutralization Buffer 35 ml
    Wash Buffer 60 ml
    Elution Buffer 20 ml
    RNase A Solution 100 μl
    Spin Columns 100 (Column Volume: 750 μl)


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