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QuickClean II Plasmid Miniprep Kit
DescriptionQuickClean II Plasmid Miniprep Kit provides a fast, simple, and cost-effective method for purification of up to 20 μg high-copy plasmid from 1â5 ml of overnight cultures of E. coli. The DNA isolated by this Kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-Time PCR and other downstream experiments.
Key FeaturesThe extracted DNA is immediately ready for routine molecular biology laboratory applications.
ApplicationsThe extracted DNA is immediately ready for further downstream applications such as the following:
ExamplesFigure 1. Plasmid DNA preparation from E. coli bearing high copy number plasmid.
The QuickClean II Plasmid Miniprep Kit is here compared to a commercially available kit (Competitor A) for rapid plasmid purification from E. coli bearing high copy number plasmid. Both kits are used in compliance with the protocols provided by the manufacturers. The results are shown in Figure 1.
M1: DL15,000 DNA Marker (Takara)
Lane 1-3: Plasmid purified by GenScript kit
Lane 4-6: Plasmid purified by Competitor A
Figure 2. Enzymatic reactions analysis
M1: DL2,000TM DNA Marker (Takara)
M2： DL15,000 DNA Marker (Takara)
Lane 2 to 9: Hind III, Hac III, Dra I, Kpn I, BamH I, Hinf I, Xho I, EcoR I