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L00430
 Protein A ELISA Kit
1 kit
$550

Protein A ELISA Kit



Description:

Protein A affinity chromatography is widely used for antibody purification. However, the leakage of Protein A, though covalently conjugated to chromatography supports, posts a big problem for antibody based pharmaceutical industry where false results could be generated in immunological assays from contaminated Protein A.

GenScript Protein A ELISA Kit is developed for accurate quantification of the leakage of native Protein A, recombinant Protein A variants and MabSelect SuRe™ in the presence or absence of antibodies. The kit offers pharmaceutical users a sensitive assay to measure mini amount of Protein A in therapeutic antibody products. It also facilitates Protein A resin manufacturers in monitoring leaching characteristics of their resins under specific conditions.


Key Features:

  • Accurate detection of 20 pg/ml Protein A and 50 pg/ml MabSelect SuRe™ in antibody preps.
  • Broad-range recongnition of native Protein A, recombinant Protein A variants and MabSelect SuRe™
  • Low level deviation - Intra-assay <5%, Inter-assay <10%
  • High accuracy/recovery within 85-115%
  • Ready-to-use ELISA plate with fast & simple samples preparation
  • Time-saving protocol - complete assay in less than 2 hours
  • Exceptional lot-to-lot consistency ensured by high-quality monoclonal antibodies
  • Wide applications for the quantification of Protein A contaminant and leaching



Storage:

  • The kit is stable for 12 months stored at 2-8°C.
  • Do not freeze the kit.

Technical Manual


Components
96-well plate coated with anti-Protein A mAb 1 Plate （12 wells x 8 strips）
Biotin conjugated anti-Protein A mAb 12 ml
Streptavidin-HRP 12 ml
Protein A Standard (10 μg/ml) 1 ml
20 × Wash Solution 40 ml
10% Tween 20 1 ml
One-Solution Microwell TMB Substrate 12 ml
Stop Solution 6 ml


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